Visualize cells according to spatial AND dimension reduction coordinates in ggplot mode
Usage
spatDimFeatPlot2D(
gobject,
spat_unit = NULL,
feat_type = NULL,
show_image = FALSE,
gimage = NULL,
image_name = NULL,
largeImage_name = NULL,
expression_values = c("normalized", "scaled", "custom"),
plot_alignment = c("vertical", "horizontal"),
feats,
order = TRUE,
dim_reduction_to_use = "umap",
dim_reduction_name = "umap",
dim1_to_use = 1,
dim2_to_use = 2,
dim_point_shape = c("border", "no_border"),
dim_point_size = 1,
dim_point_alpha = 1,
dim_point_border_col = "black",
dim_point_border_stroke = 0.1,
show_NN_network = FALSE,
show_spatial_network = FALSE,
dim_network_color = "gray",
nn_network_to_use = "sNN",
network_name = "sNN.pca",
dim_edge_alpha = NULL,
scale_alpha_with_expression = FALSE,
sdimx = "sdimx",
sdimy = "sdimy",
spatial_network_name = "Delaunay_network",
spatial_network_color = NULL,
show_spatial_grid = FALSE,
grid_color = NULL,
spatial_grid_name = "spatial_grid",
spat_point_shape = c("border", "no_border", "voronoi"),
spat_point_size = 1,
spat_point_alpha = 1,
spat_point_border_col = "black",
spat_point_border_stroke = 0.1,
spat_edge_alpha = NULL,
cell_color_gradient = NULL,
gradient_midpoint = NULL,
gradient_style = c("divergent", "sequential"),
gradient_limits = NULL,
cow_n_col = NULL,
cow_rel_h = 1,
cow_rel_w = 1,
cow_align = "h",
show_legend = TRUE,
legend_text = 10,
dim_background_color = "white",
spat_background_color = "white",
vor_border_color = "white",
vor_max_radius = 200,
vor_alpha = 1,
axis_text = 8,
axis_title = 8,
show_plot = NULL,
return_plot = NULL,
save_plot = NULL,
save_param = list(),
default_save_name = "spatDimFeatPlot2D"
)Arguments
- gobject
giotto object
- spat_unit
spatial unit (e.g. "cell")
- feat_type
feature type (e.g. "rna", "dna", "protein")
- show_image
show a tissue background image
- gimage
a giotto image
- image_name
name of a giotto image or multiple images with group_by
- largeImage_name
deprecated. Use
image_name- expression_values
feat expression values to use
- plot_alignment
direction to align plot
- feats
features to show
- order
order points according to feature expression
- dim_reduction_to_use
character. dimension reduction to use
- dim_reduction_name
character. dimension reduction name
- dim1_to_use
numeric. dimension to use on x-axis
- dim2_to_use
numeric. dimension to use on y-axis
- dim_point_shape
point with border or not (border or no_border)
- dim_point_size
size of points in dim. reduction space
- dim_point_alpha
transparancy of point in dim. reduction space
- dim_point_border_col
border color of points in dim. reduction space
- dim_point_border_stroke
border stroke of points in dim. reduction space
- show_NN_network
logical. Show underlying NN network
- show_spatial_network
show spatial network
- dim_network_color
color of NN network
- nn_network_to_use
character. type of NN network to use (kNN vs sNN)
- network_name
name of NN network to use, if show_NN_network = TRUE
- dim_edge_alpha
dim reduction plot: column to use for alpha of the edges
- scale_alpha_with_expression
scale expression with ggplot alpha parameter
- sdimx
spatial x-axis dimension name (default = 'sdimx')
- sdimy
spatial y-axis dimension name (default = 'sdimy')
- spatial_network_name
name of spatial network to use
- spatial_network_color
color of spatial network
- show_spatial_grid
show spatial grid
- grid_color
color of spatial grid
- spatial_grid_name
name of spatial grid to use
- spat_point_shape
spatial points with border or not (border or no_border)
- spat_point_size
spatial plot: point size
- spat_point_alpha
transparency of spatial points
- spat_point_border_col
color of border around points
- spat_point_border_stroke
stroke size of border around points
- spat_edge_alpha
edge alpha
- cell_color_gradient
character. continuous colors to use. palette to use or vector of colors to use (minimum of 2).
- gradient_midpoint
numeric. midpoint for color gradient
- gradient_style
either 'divergent' (midpoint is used in color scaling) or 'sequential' (scaled based on data range)
- gradient_limits
numeric vector with lower and upper limits
- cow_n_col
cowplot param: how many columns
- cow_rel_h
cowplot param: relative heights of rows (e.g. c(1,2))
- cow_rel_w
cowplot param: relative widths of columns (e.g. c(1,2))
- cow_align
cowplot param: how to align
- show_legend
logical. show legend
- legend_text
size of legend text
- dim_background_color
color of plot background for dimension plot
- spat_background_color
color of plot background for spatial plot
- vor_border_color
border colorr for voronoi plot
- vor_max_radius
maximum radius for voronoi 'cells'
- vor_alpha
transparancy of voronoi 'cells'
- axis_text
size of axis text
- axis_title
size of axis title
- show_plot
logical. show plot
- return_plot
logical. return ggplot object
- save_plot
logical. save the plot
- save_param
list of saving parameters, see
showSaveParameters- default_save_name
default save name for saving, don't change, change save_name in save_param
Examples
g <- GiottoData::loadGiottoMini("visium", verbose = FALSE)
spatDimFeatPlot2D(g, feats = c("Gna12", "Ccnd2", "Btbd17"))
