Read a 10x image that is provided with an affine matrix transform. Loads the image in with an orientation that matches the dataset points and polygons vector information

read10xAffineImage(
  file,
  imagealignment_path,
  name = "aligned_image",
  micron = 0.2125,
  ...
)

Arguments

file

filepath to image

imagealignment_path

filepath to alignment file which contains an affine transformation matrix. Usually a .csv file

name

character. Name to assign the image. Default is 'image'.

micron

micron scaling. Directly used if a numeric is supplied. Also prefers a filepath to the experiment.xenium file which contains this info. A default of 0.2125 is provided.

...

additional params to pass to [GiottoClass::createGiottoLargeImage]

Value

10xAffineImage